Biochemistry and molecular biology*

The 15th Iranian National Congress of Biochemistry & 6th International Congress of Biochemistry and Molecular Biology

Genetic diversity analysis of aquaculture strains of Acipenser stellatus (Pallas, 1771) using DNA markers

Acipenser stellatus (stellate sturgeon) represents a sturgeon species of great importance for the scientific community and fishermen that has been intensively captured for decades. Besides of overfishing and poaching, their reproduction sites have been destroyed and their natural habitats were affected by pollution. In consequence, wild populations of stellate sturgeon have decreased dramatical...

Genetic diversity analysis of aquaculture strains of Acipenser stellatus (Pallas, 1771) using DNA markers

Acipenser stellatus (stellate sturgeon) that has been intensively captured for decades represents a sturgeon species of great importance for the scientific community and fishermen. Besides overfishing and poaching, their reproduction sites have been destroyed and their natural habitats were affected by pollution. In consequence, wild populations of stellate sturgeon have decreased dramatically ...

Quantitation of Vascular Endothelial Growth Factor and Interleukin-6 in Different Stages of Breast Cancer

Background: Determination of the impact of angiogenesis on tumor development and progression is essential. This study aimed to determine the serum levels of Vascular endothelial growth factor (VEGF) and Interleukin 6 (IL-6) in breast carcinoma, and to correlate them with tumor size, lymph node involvement, and cancer stage. Methods: Under aseptic precautions 5 ml of venous blood was collecte...

The downregulation of ATG4B mediated by microRNA-34a/34c-5p suppresses rapamycin-induced autophagy

Objective(s): Autophagy-related 4B (ATG4B) plays an important role in the process of autophagy induction. However, the molecular events that govern the expression of ATG4B in this process are not well known. Materials and Methods: Human ATG4B 3'-UTR region (1377 nt) containing miR-34a/miR-34c-5p binding site was amplified by PCR. Luciferase assay was used to assess the activity of reporter gene...

Fifty Percent Ethanolic Extract of Momordica charantia Inhibits Adipogenesis and Promotes Adipolysis in 3T3-L1 Pre-Adipocyte Cells

Background: Natural products have gained importance recently for the treatment of obesity and its complications, partly because of the side effects of modern drugs.Hence, we aimed to study and compare the effect of varying concentrations of Momordicacharantiaon adipogenesis and adipolysis using 3T3-L1 pre-adipocyte cell lines. Methods: 3T3-L1 pre-adipocytes were procured from the National Ce...